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firefly luciferase reporter plasmid pkm53  (Addgene inc)


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    Addgene inc firefly luciferase reporter plasmid pkm53
    Firefly Luciferase Reporter Plasmid Pkm53, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/luciferase+reporter+plasmids/pKM53+(Plasmid+%23154156)/pm41888882-90-12-17
    Average 94 stars, based on 1 article reviews
    firefly luciferase reporter plasmid pkm53 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Luciferase:

    Article Title: Expression of HIF1α in intestinal epithelium restricts arthritis inflammation by inhibiting RIPK3-induced cell death machinery
    Article Snippet: The recombinant vectors were sequenced in Microsynth Seqlab (Göttingen, Germany) to validate the presence of inserted HREs. .. MC38 cells were co-transfected with luciferase reporter plasmids, HIF1α or HIF2α TM plasmids (Addgene) and Renilla plasmids (Addgene) using the Lipofectamine 3000 kit (Invitrogen). ..

    Article Title: Δ133p53α and Δ160p53α isoforms of the tumor suppressor protein p53 exert dominant-negative effect primarily by co-aggregation
    Article Snippet: .. Luciferase reporter plasmids, WWP/p21-Luc (RRID: Addgene_16451 ) , pGL3-MDM2-Luc (RRID: Addgene_32369 ) , and PUMA Frag1-Luc (RRID: Addgene_16591 ) ( ) were obtained from Addgene. ..

    Article Title: Regulation of KCNMA1 transcription by Nrf2 in coronary arterial smooth muscle cells.
    Article Snippet: KCNMA1 luciferase reporter plasmids (pEZX-PG02-BKα-Luc, product ID: HPRM32206) containing a segment of the human KCNMA1 promotor (−1369~ − 1 oligonucleotides, NM_001014797) with one putative ARE-Nrf2 binding site [(−857)TGAGTCCGC(−849)], and KCNMB1 luciferase reporter plasmids (pEZX-PG02-BKβ1-Luc, product ID: HPRM45337) containing a segment of the human KCNMβ1 promotor (−5481~−4161 oligonucleotides, NM_004137) with one putative ARE-Nrf2 binding site [(−5449) TGAGCTCGC (−5441)] were purchased from GeneCopoeia, Inc. (Rockville, MD, USA). .. The luciferase reporter plasmids were co-transfected with pcDNA3.1 plasmids containing Nrf2 gene (pcDNA3.1-Nrf2) (Addgene, Inc.) into HEK293 cells. ..

    Article Title: Δ133p53 and Δ160p53 isoforms of the tumor suppressor protein p53 exert dominant-negative effect primarily by co-aggregation
    Article Snippet: .. Luciferase reporter plasmids, WWP/p21-Luc (Plasmid #16451) , pGL3-MDM2-Luc (Plasmid #32365) , and PUMA Frag1-Luc (Plasmid #16591) were obtained from Addgene. ..

    Plasmid Preparation:

    Article Title: Δ133p53 and Δ160p53 isoforms of the tumor suppressor protein p53 exert dominant-negative effect primarily by co-aggregation
    Article Snippet: .. Luciferase reporter plasmids, WWP/p21-Luc (Plasmid #16451) , pGL3-MDM2-Luc (Plasmid #32365) , and PUMA Frag1-Luc (Plasmid #16591) were obtained from Addgene. ..



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    ABLIM1 is a downstream target of miR-378a-5p and involves in abdominal aortic aneurysm development. (A) Volcano plot of differently expressed genes in GSE183464 and GSE237229 database. Venn diagram showed intersection of differentially expressed genes and predicted target genes including ABLIM1 , DDX5 and SLC7A1 . (B) RT-qPCR analysis of target genes Ablim1 , Ddx5 and Slc7a1 in the TNFα-treated vascular smooth muscle cells (n=3 per group). RT-qPCR analysis of Ablim1 , Ddx5 and Slc7a1 in the aortas of Ang II-treated mice (n=3 per group). (C) ABLIM1 expression in the aortas treated-with antagomir-NC or antagomir-378a-5p was identified using by immunofluorescence staining. ABLIM1 (red), α-SMA (green) and DAPI (blue). (D) Representative images of immunofluorescence staining identified ABLIM1 expression in aortas with angomir-NC or angomir-378a-5p. ABLIM1 (red), α-SMA (green) and DAPI (blue). (E) Conservatism analysis of the binding site for miR-378a-5p and ABLIM1 in in humans, mice and rat. (F) Luciferase activity in 293T cells transfected with mimics-miR-378a-5p together with ABLIM1 -3'UTR-wild type or mutant plasmid. Data are presented as the mean ± SEM. P-values were calculated by Student's t test (for B). ** P<0.01 vs. Control or saline or mimics-NC- ABLIM1 -3'UTR-WT. ABLIM1, actin-binding LIM protein 1; miR or miRNA, microRNA; RT-qPCR, reverse transcription-quantitative PCR; α-SMA, α-smooth muscle actin; NC, negative control; UTR, untranslated region; WT, wild-type; MUT, mutated.

    Journal: International Journal of Molecular Medicine

    Article Title: miRNA-378a-5p attenuates the development of abdominal aortic aneurysm via ABLIM1-MKL1 signaling pathways

    doi: 10.3892/ijmm.2026.5768

    Figure Lengend Snippet: ABLIM1 is a downstream target of miR-378a-5p and involves in abdominal aortic aneurysm development. (A) Volcano plot of differently expressed genes in GSE183464 and GSE237229 database. Venn diagram showed intersection of differentially expressed genes and predicted target genes including ABLIM1 , DDX5 and SLC7A1 . (B) RT-qPCR analysis of target genes Ablim1 , Ddx5 and Slc7a1 in the TNFα-treated vascular smooth muscle cells (n=3 per group). RT-qPCR analysis of Ablim1 , Ddx5 and Slc7a1 in the aortas of Ang II-treated mice (n=3 per group). (C) ABLIM1 expression in the aortas treated-with antagomir-NC or antagomir-378a-5p was identified using by immunofluorescence staining. ABLIM1 (red), α-SMA (green) and DAPI (blue). (D) Representative images of immunofluorescence staining identified ABLIM1 expression in aortas with angomir-NC or angomir-378a-5p. ABLIM1 (red), α-SMA (green) and DAPI (blue). (E) Conservatism analysis of the binding site for miR-378a-5p and ABLIM1 in in humans, mice and rat. (F) Luciferase activity in 293T cells transfected with mimics-miR-378a-5p together with ABLIM1 -3'UTR-wild type or mutant plasmid. Data are presented as the mean ± SEM. P-values were calculated by Student's t test (for B). ** P<0.01 vs. Control or saline or mimics-NC- ABLIM1 -3'UTR-WT. ABLIM1, actin-binding LIM protein 1; miR or miRNA, microRNA; RT-qPCR, reverse transcription-quantitative PCR; α-SMA, α-smooth muscle actin; NC, negative control; UTR, untranslated region; WT, wild-type; MUT, mutated.

    Article Snippet: The 3' untranslated region (3'UTR) of mouse Ablim1 , Ddx5 (Dead-box helicase 5), Slc7a1 (Cationic amino acid transporter 1) gene was amplified and cloned into the pMIR-REPORT Luciferase (OBiO Technology Company) to construct pMIR-REPORT Luciferase 3'UTR wild-type (WT) plasmids.

    Techniques: Quantitative RT-PCR, Expressing, Immunofluorescence, Staining, Binding Assay, Luciferase, Activity Assay, Transfection, Mutagenesis, Plasmid Preparation, Control, Saline, Reverse Transcription, Real-time Polymerase Chain Reaction, Negative Control